Shoots Multiplication of Tawangmangu Orange (Citrus nobilis L.) With Variation of Iba and Kinetin Concentrations

Oleh : Syariffah Nur Aini, Samanhudi, and Ahmad Yunus

Kategori : Journal Agronomy Research

Volume :  Vol 1 No 1: 7-14, Oktober 2012

Abstract :

Tawangmangu Orange (Citrus nobilis L.) is a potential commodity to be developed however constrained with its existence was a little. While large scale industry requires large amounts of many seedlings, same and fast. One solution is duplication by in vitro. This research was conducted in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, Sebelas Maret University Surakarta from Januari 2012 until Juli 2012, used factorial design with two factors based on Completely Randomized Design. The first factor was IBA concentrations consist of four levels: 0; 0.5; 1; 1.5 ppm and the second factor was kinetin concentrations consist four levels: 0.5; 1; 1.5; 2 ppm. There were 16 combination treatments, each of them was repeated for three times. The results showed that the interaction w as not occur between IBA and kinetin. Combination treatment of 1.5 ppm IBA and 1 ppm kinetin produced the largest number of shoots (2.33 shoots) and produced the largest number of leaves (2.33 leaves). The best of shoots high at combination 1 ppm IBA and 2 ppm kinetin (8.33 mm).

Keywords: Citrus nobilis L., multiplication, IBA, kinetin