Category Archives: Volume 1 No. 1 : Oktober 2012

Exploration of Bacteriophage Virulent to Xanthomonas campestris pv. Campestris Isolated from Tawangmangu for Controlling Cabbage Black Rot Disease

Oleh : Sri Widadi, Supyani, Sumijati and Yulia Rahmawati

Kategori : Journal Agronomy Research

Volume :  Vol 1 No 1: 15-19, Oktober 2012

Abstract :

The aims of this study are to explore and learn bacteriophage ability to control black rot disease of cabbage. This study was conducted in March through July 2012. The research was conducted in two phases: (1) bacteriophage isolation by plaque assay, (2) testing bacteriophage to control black rot of cabbage in the field. The results showed that bacteriophage can be isolated from various sources that  cabbage leaves, diseased cabbage root, and diseased cabbage soil. The amount of bacteriophage (based on plaque assay) at most of the diseased cabbage root. For the observation incidence and severity, control treatments is highest the incidence and severity of disease than other treatments (with application bacteriophage). Because without the bacteriophage, Xanthomonas campestris pv. campestris can replicate and cause symptoms of black rot of cabbage. This indicates that application bacteriophage able to suppress the attack of black rot disease of cabbage.

Keywords: bacteriophage, black rot, cabbage, Xanthomonas campestris pv. campestris, Tawangmangu


Shoots Multiplication of Tawangmangu Orange (Citrus nobilis L.) With Variation of Iba and Kinetin Concentrations

Oleh : Syariffah Nur Aini, Samanhudi, and Ahmad Yunus

Kategori : Journal Agronomy Research

Volume :  Vol 1 No 1: 7-14, Oktober 2012

Abstract :

Tawangmangu Orange (Citrus nobilis L.) is a potential commodity to be developed however constrained with its existence was a little. While large scale industry requires large amounts of many seedlings, same and fast. One solution is duplication by in vitro. This research was conducted in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, Sebelas Maret University Surakarta from Januari 2012 until Juli 2012, used factorial design with two factors based on Completely Randomized Design. The first factor was IBA concentrations consist of four levels: 0; 0.5; 1; 1.5 ppm and the second factor was kinetin concentrations consist four levels: 0.5; 1; 1.5; 2 ppm. There were 16 combination treatments, each of them was repeated for three times. The results showed that the interaction w as not occur between IBA and kinetin. Combination treatment of 1.5 ppm IBA and 1 ppm kinetin produced the largest number of shoots (2.33 shoots) and produced the largest number of leaves (2.33 leaves). The best of shoots high at combination 1 ppm IBA and 2 ppm kinetin (8.33 mm).

Keywords: Citrus nobilis L., multiplication, IBA, kinetin

Potency of Se-NPV In Controling Spodoptera Exigua on Shallot

Oleh : Pratiwi Noviayanti, Retno Wijayanti and Supyani

Kategori : Journal Agronomy Research

Volume :  Vol 1 No 1: 57-63, Oktober 2012

Abstract :

Beet army worm (BAW) (Spodoptera exigua Hubn.) has big enough damage on shallot cultivations. Management always use chemical insecticide that results adverse effect on consumer and ecosystem. Se-NPV is one of biological control agents that can control BAW. This research aimed to study the potency of Se-NPV in controling BAW and compare it with another NPV isolate (Ms-NPV) on BAW. Research carried out Completely Randomized Design method consist of 6 treatments and had been repeated four times. Research was conducted by laboratory testing (mortality, biology, larval eating ability and pupal weight) and field testing (damage intensity and field mortality). The results showed that application of Se-NPV and Ms-NPV were effective enough in controling S. exigua with the highest larval mortality at 77,5% for Se-NPV and 95% for Ms-NPV and depressed its damage intensity on shallots with the lowest intensity at 10,43%. NPV application can also affect larval period, pupal weight and adult emergence. NPV’s infection did not affect pupal period and adult period.

Key words: Se-NPV, Spodoptera exigua, shallot, biological control.

Application of Auksin (2,4-D) And Cytokinin (BAP) As Inducer of Mabai (Pongamia pinnata) Callus by In Vitro

Oleh : Martha Dwi Jayanti, Samanhudi, and Parjanto

Kategori : Journal Agronomy Research

Volume :  Vol 1 No 1: 1-6, Oktober 2012

Abstract :

Human depends of fossil fuels can cause global warming. Therefore, the need for alternative fuels such as mabai. Mabai has its own advantages in terms of raw material supply biodiesel because it does not be a competitor with edible oil, the oil extraction process is easy, and good for environment. Provision of seeds in large quantities in a short time needs to be done by using the technique in vitro by adding 2,4-D and BAP. This study aims to obtain the concentration of 2,4-D and BAP that most appropriate in the formation of callus mabai in vitro culture. This research was held in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, University of Sebelas Maret Surakarta from November 2011 until July 2012. It used factorial design with two factors and repeated for three times based on Completely Randomized Design. The first factor was 2,4-D concentrations with four levels: 0.25, 0.5, 0.75, 1 ppm and the second factor was BAP concentrations with five levels: 0, 0.5, 1, 1.5, 2 ppm. The observation data were analyzed with analysis of variance and if significant continued with DMRT test at 5% level. If there was a data that could not be calculated, it would be analyzed using descriptive statistics. The results showed that there was no interaction between 2,4-D and BAP. Treatment of 2,4-D 0.25 ppm and BAP 0 ppm induced callus with the fastest time (10 DAP). Four treatments that produce callus has a compact texture. The best treatment was came from 2,4-D 0.75 ppm and BAP 1.5 ppm that produced green callus.

Key word : Pongamia pinnata; 2,4-D; BAP; in vitro

The Dynamics of N2O Emission In Rice Farming Land with Fertilization and Water Management System Variation in Demakan Village, Mojolaban Subdistrict, Sukoharjo

Oleh : Maria Niken Puri Andari, Purwanto, and Djoko Mursito

Kategori : Journal Agronomy Research

Volume :  Vol 1 No 1: 49-56, Oktober 2012

Abstract :

This research aimed at determining the effect of water stress intensity on the growth, yield and anthocyanin content of Light Red and Dark Red Roselle calyxes. The research was conducted from January to June 2012 at Greenhouse of Faculty of Agriculture, University of March Surakarta. The research design used Randomized Complete Design in factorial arranged. The factors are varieties (Light Red Roselle and Dark Red Roselle), and water stress intensity (100% of field capacity/control, 75% of field capacity, 50% of field capacity, and 25% of field capacity). The results showed that water stress reduce the plant height, fresh weight of calyxes and dry weight of calyxes in both Light Red and Dark Red Roselle. The highest of anthocyanin content taken from calyxes of Light Red Roselle were result at 75% of field capacity (23,34 mg/100 g), while the Dark Red Roselle were resulted in the control/field capacity condition (174,69 mg/100 g) and decreased with increasing water stress intensity.

 Keywords: water stress, Light Red RoselleDark Red Roselleanthocyanin