Application of Auksin (2,4-D) And Cytokinin (BAP) As Inducer of Mabai (Pongamia pinnata) Callus by In Vitro

Oleh : Martha Dwi Jayanti, Samanhudi, and Parjanto

Kategori : Journal Agronomy Research

Volume :  Vol 1 No 1: 1-6, Oktober 2012

Abstract :

Human depends of fossil fuels can cause global warming. Therefore, the need for alternative fuels such as mabai. Mabai has its own advantages in terms of raw material supply biodiesel because it does not be a competitor with edible oil, the oil extraction process is easy, and good for environment. Provision of seeds in large quantities in a short time needs to be done by using the technique in vitro by adding 2,4-D and BAP. This study aims to obtain the concentration of 2,4-D and BAP that most appropriate in the formation of callus mabai in vitro culture. This research was held in Plant Physiology and Biotechnology Laboratory of Agriculture Faculty, University of Sebelas Maret Surakarta from November 2011 until July 2012. It used factorial design with two factors and repeated for three times based on Completely Randomized Design. The first factor was 2,4-D concentrations with four levels: 0.25, 0.5, 0.75, 1 ppm and the second factor was BAP concentrations with five levels: 0, 0.5, 1, 1.5, 2 ppm. The observation data were analyzed with analysis of variance and if significant continued with DMRT test at 5% level. If there was a data that could not be calculated, it would be analyzed using descriptive statistics. The results showed that there was no interaction between 2,4-D and BAP. Treatment of 2,4-D 0.25 ppm and BAP 0 ppm induced callus with the fastest time (10 DAP). Four treatments that produce callus has a compact texture. The best treatment was came from 2,4-D 0.75 ppm and BAP 1.5 ppm that produced green callus.

Key word : Pongamia pinnata; 2,4-D; BAP; in vitro